What is the significance of the reading frame

And "open" means that the road is open to keep reading, and the ribosome will be able to keep reading the RNA code and add another amino acid one after another. And when it's translated into a protein, the mRNA is not read one letter at a time, but it's read three letters at a time. And those three letters are called a codon, and each of those codons, whether it's an AAA or UUU or an AUG, each of those codons is interpreted by the ribosome, the molecular machine, that's going to make the protein as a certain amino acid.

So an open reading frame is the length of DNA, or RNA, which is transcribed into RNA, through which the ribosome can travel, adding one amino acid after another before it runs into a codon that doesn't code for any amino acid.

And when that happens, it confuses the ribosome, and the ribosome stops. So you'll be pleased to hear that codons, which make that happen are called stop codons, and a stop codon ends an open reading frame. With pemission from Dermatology Atlas. Since DNA has two anti-parallel strands, an additional three reading frames arise, giving a possible six frame translations. Template:WikiDoc Sources. Significance One common use of open reading frames is as one piece of evidence to assist in gene prediction.

Diagnosis Physical Examination Skin Extremity. Computational Genome Analysis: an introduction. ISBN Categories : Pages with citations using unsupported parameters Molecular genetics. Cookies help us deliver our services. By using our services, you agree to our use of cookies. An open reading frame starts with an atg Met in most species and ends with a stop codon taa , tag or tga.

For example, the following sequence of DNA can be read in six reading frames. Three in the forward and three in the reverse direction. The three reading frames in the forward direction are shown with the translated amino acids below each DNA seqeunce.

Frame 1 starts with the " a ", Frame 2 with the " t " and Frame 3 with the " g ". The longest ORF is in Frame 1. DNA Translation. Select Nucleic Tools and then Select the sequence that you wish to translate by checking the box next to the file. You will be given a box asking you to select various parameters.